Louis Hébert visited Canada three times between 1604 and 1613 with the expeditions of Pierre Degua de Monts, Samuel de Champlain, and Jean de Biencourt de Pourtrincourt. In 1617 he decided to settle in Québec City with his wife and three children. He was ceded 10 arpents of land near the site of the present cathedral of Québec, and his family is renowned for having been the first to cultivate land in Canada.

His wife, Marie Rollet, was the first Frenchwoman to scratch the soil of New France. It is said that, thanks to his medical knowledge among other reasons, Hébert was on good terms with the native people.